pa1957  (Boster Bio)

Journal: Cell reports

Article Title: p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP

doi: 10.1016/j.celrep.2023.113486

Figure Lengend Snippet:

Article Snippet: ZO-2 , rabbit polyclonal , Boster , PA1957.

Techniques:

Journal: Cell reports

Article Title: p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP

doi: 10.1016/j.celrep.2023.113486

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ZO-2 , rabbit polyclonal , Boster , PA1957.

Techniques: Virus, Recombinant, cDNA Synthesis, RNA Sequencing, Knockdown, Plasmid Preparation, shRNA, Software

Journal: Cell reports

Article Title: p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP

doi: 10.1016/j.celrep.2023.113486

Figure Lengend Snippet: (A) Validation of the interaction between p190A and ZO-1/2 detected by mass spectrometry. Confluent cultures of control cells and H661 expressing Myc-tagged p190A cells were lysed in gold lysis buffer and processed for immunoprecipitation (IP) using mouse monoclonal 9E10 anti-Myc epitope antibody immobilized on agarose beads followed by western blotting (WB) to detect p190A, ZO-1, ZO-2, and ERK1/2. (B) Confluent cultures of MDA-MB-231 cells were processed for immunoprecipitation with rabbit polyclonal anti-p190A antibody or without antibody followed by protein A/G Sepharose beads. Next, p190A, RasGAP, ZO-1, ZO-2, and ERK1/2 were detected in immunoprecipitates and lysates by western blotting. (C) ZO-1 and ZO-2 bind p190A in a RasGAP-dependent manner. Confluent cultures of control cells or H661 cells expressing Myc-tagged p190A(WT) ± RASA1-KO or p190A(Y2F) were processed for immunoprecipitation with 9E10 antibody followed by western blotting to detect p190A, RasGAP, ZO-1, ZO-2, and ERK1/2. (D) Binding of p190A to RasGAP is independent of ZO-2. Confluent cultures of control cells or H661 cells expressing Myc-tagged p190A ± TJP2-KO were processed for immunoprecipitation with 9E10 antibody followed by western blotting to detect p190A, RasGAP, ZO-2, and ERK1/2. (E and F) The U5GuK and ABR domains of ZO-1 are necessary for the interaction with p190A. (E) Cartoon depicting domain structure of zonula occludens proteins ZO-1, ZO-2, and ZO-3. The regions encoded by ZO-1 and ZO-2 sequences targeted by sgRNAs are marked. (F) MDCK II cells depleted of endogenous ZO-1 and expressing exogenous EGFP only or knockdown-resistant EGFP-tagged full-length ZO-1 or ZO-1 deletion mutant missing the ABR, U5GuK, or PDZ1 domains were processed for immunoprecipitation with polyclonal rabbit anti-p190A antibody followed by western blotting to detect GFP, p190A, and ERK1/2. (G) Interaction of ZO-2 with p190A in MDCK cells. MDCK II cells depleted of endogenous ZO-2 and expressing exogenous EGFP only or knockdown-resistant EGFP-tagged full-length ZO-2 were processed for immunoprecipitation with polyclonal p190A antibody followed by western blotting to detect GFP and p190A. (H) Immunofluorescence to detect p190A (red) in MDCK II cell depleted of ZO-1 or ZO-2 expressing either EGFP alone or knockdown-resistant EGFP-tagged full-length ZO-1 or ZO-2 as indicated. Scale bars represent 10 μm. (I) 5× magnification of the boxed areas in (H).

Article Snippet: ZO-2 , rabbit polyclonal , Boster , PA1957.

Techniques: Biomarker Discovery, Mass Spectrometry, Control, Expressing, Lysis, Immunoprecipitation, Western Blot, Binding Assay, Knockdown, Mutagenesis, Immunofluorescence

Journal: Cell reports

Article Title: p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP

doi: 10.1016/j.celrep.2023.113486

Figure Lengend Snippet:

Article Snippet: ZO-2 , rabbit polyclonal , Boster , PA1957.

Techniques: